WB result of CD100/Semaphorin-4D Recombinant Rabbit mAb
Primary antibody: CD100/Semaphorin-4D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 100-150 kDa
CD100/Semaphorin-4D Recombinant Rabbit mAb (S-3651-16)
CD100/Semaphorin-4D Recombinant Rabbit mAb (S-3651-16)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD100/Semaphorin-4D |
| Synonyms | Semaphorin-4D; A8; BB18; GR3; C9orf164; SEMAJ; SEMA4D |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q92854 |
| Clone Number | S-3651-16 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Daudi, Jurkat, EL4, mouse thymus, rat thymus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
CD100, also known as Semaphorin-4D (Sema4D), is a type I transmembrane glycoprotein belonging to the semaphorin family that functions as a critical bidirectional signaling molecule in both the nervous and immune systems. Originally identified for its role in axon guidance and neuronal development by repelling growth cones through its interaction with Plexin-B1 receptors, CD100 has since been recognized as a potent immunomodulator expressed on activated T cells, B cells, dendritic cells, and various tumor types. Upon cleavage by metalloproteinases, it releases a soluble form that retains biological activity, engaging primarily with Plexin-B1 on endothelial and tumor cells to promote angiogenesis, cell migration, and invasion, while also binding to CD72 on B cells to regulate their activation and differentiation. This dual functionality makes CD100/Sema4D a pivotal player in pathological processes such as tumor progression, metastasis, and inflammatory responses, thereby establishing it as a promising therapeutic target for cancer immunotherapy and autoimmune disease management.
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Western Blot
WB result of CD100/Semaphorin-4D Recombinant Rabbit mAb
Primary antibody: CD100/Semaphorin-4D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: EL4 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 110-150 kDa
WB result of CD100/Semaphorin-4D Recombinant Rabbit mAb
Primary antibody: CD100/Semaphorin-4D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse colon lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Low expression control: mouse colon lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 110-150 kDa
WB result of CD100/Semaphorin-4D Recombinant Rabbit mAb
Primary antibody: CD100/Semaphorin-4D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat colon lysate 20 µg
Lane 2: rat thymus lysate 20 µg
Low expression control: rat colon lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 90-150 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-CD100/Semaphorin-4D antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD100/Semaphorin-4D antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD100/Semaphorin-4D antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD100/Semaphorin-4D antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CD100/Semaphorin-4D antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti- CD100/Semaphorin-4D antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti- CD100/Semaphorin-4D antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
