WB result of CCR8 Recombinant Rabbit mAb
Primary antibody: CCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HuT 78 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
CCR8 Recombinant Rabbit mAb (S-1414-148)
CCR8 Recombinant Rabbit mAb (S-1414-148)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CCR8 |
| Synonyms | C-C chemokine receptor type 8; C-C CKR-8; CC-CKR-8; CCR-8; CC chemokine receptor CHEMR1; CMKBRL2; Chemokine receptor-like 1 (CKR-L1); GPR-CY6 (GPRCY6); TER1; CDw198; CKRL1; CMKBR8; CMKBRL2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P51685 |
| Clone Number | S-1414-148 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms |
| Positive Sample | A431, HuT 78, mouse spleen, mouse thymus |
| Predicted Reactivity | Mk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IP | 1:50 | Hu |
Background
CCR8 (C-C motif chemokine receptor 8) is a chemokine receptor that plays a significant role in immune cell trafficking and is highly expressed on regulatory T cells (Tregs) in the tumor microenvironment. In the context of cancer, CCR8 is specifically highly expressed on Tregs within the tumor tissue, but is minimally expressed in peripheral blood and normal tissues, making it a specific biomarker for Tregs at the tumor site. Its signaling pathway is involved in the regulation of various solid tumors, such as gastric cancer, breast cancer, and colorectal cancer, among others. Therefore, CCR8 is considered a highly potential target for tumor immunotherapy.
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Picture
Western Blot
WB result of CCR8 Recombinant Rabbit mAb
Primary antibody: CCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse thymus lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
IP
CCR8 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CCR8 in 0.4 mg HuT 78 whole cell lysate.
Western blot was performed on the immunoprecipitate using CCR8 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HuT 78 whole cell lysate 20 µg (Input)
Lane 2: CCR8 Rabbit mAb IP in HuT 78 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HuT 78 whole cell lysate
Predicted MW: 41 kDa
Observed MW: 45 kDa
