WB result of CCL18 Recombinant Rabbit mAb
Primary antibody: CCL18 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 0.1 µg/ml PMA for 48 hours, then add 0.045 µg/ml hIL-4 for 72 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 11 kDa
CCL18 Recombinant Rabbit mAb (S-2788-25)
CCL18 Recombinant Rabbit mAb (S-2788-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CCL18 |
| Synonyms | C-C motif chemokine 18; Alternative macrophage activation-associated CC chemokine 1 (AMAC-1); CC chemokine PARC; Dendritic cell chemokine 1 (DC-CK1); Macrophage inflammatory protein 4 (MIP-4); Pulmonary and activation-regulated chemokine; Small-inducible cytokine A18; AMAC1; DCCK1; MIP4; PARC; SCYA18 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P55774 |
| Clone Number | S-2788-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu |
| Positive Sample | PMA and hIL-4 treated THP-1 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| IF | 1:100 | Hu |
Background
CCL18 (also known as PARC, DC-CK1, AMAC-1, or MIP-4) is an 8-kDa, primate-specific CC-chemokine consisting of 89 amino acids (with a 20-residue N-terminal signal peptide cleaved to yield the 69-aa mature form) that lacks a known rodent orthologue and whose receptor remains unidentified despite extensive research. Secreted predominantly by monocytes/macrophages and dendritic cells—especially M2 tumor-associated macrophages—CCL18 acts as a chemoattractant for lymphocytes and immature dendritic cells while also functioning as a neutral CCR3 antagonist and binding promiscuously to PITPNM3, GPR30, and CCR8. Constitutively present at high levels in human plasma, CCL18 contributes to physiological lymphocyte and DC homing and primary immune responses, yet its production is markedly up-regulated in numerous pathologies, including cancers (e.g., ovarian carcinoma) and inflammatory disorders of joints, lungs, and skin. Elevated circulating CCL18 correlates with adverse outcomes such as poor survival in ovarian cancer and increased mortality in idiopathic pulmonary fibrosis, highlighting its potential as a biomarker and therapeutic target [^11].
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CCL18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CCL18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CCL18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CCL18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CCL18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CCL18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-CCL18 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
