WB result of Atg5 Recombinant Rabbit mAb
Primary antibody: Atg5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: Saos-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 56 kDa
Atg5 Recombinant Rabbit mAb (S-3181)
Atg5 Recombinant Rabbit mAb (S-3181)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Atg5 |
| Synonyms | Autophagy protein 5; APG5-like; Apoptosis-specific protein; APG5L; ASP; ATG5 |
| Location | Cytoplasm |
| Accession | Q9H1Y0 |
| Clone Number | S-3181 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | PANC-1, U-87 MG, HCT 116, MCF7, HeLa, Saos-2, MEF, C2C12, C6, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
Autophagy-related protein 5 (ATG5), encoded by the ATG5 gene on chromosome 6, is a core autophagy protein that forms a complex with ATG12 and ATG16L1 (the Atg12-Atg5-Atg16 complex), which is essential for lipidation of LC3 (microtubule-associated protein 1 light chain 3B) and subsequent autophagosome elongation and maturation. Beyond autophagy, ATG5 also has non-autophagic roles, including promoting apoptosis via mitochondrial translocation and cytochrome c release, regulating immune responses (e.g., antigen presentation and lymphocyte development), and affecting hematopoietic stem cell maintenance. ATG5 deficiency (e.g., in knockout mice) is neonatal lethal, causing autophagy defects, neurodegeneration, and impaired fertility, while its overexpression extends lifespan. Additionally, ATG5 mutations are linked to human diseases (e.g., ataxia), and it plays a role in viral infections (e.g., HIV-1) by modulating host antiviral responses.
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Western Blot
WB result of Atg5 Recombinant Rabbit mAb
Primary antibody: Atg5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 56 kDa
WB result of Atg5 Recombinant Rabbit mAb
Primary antibody: Atg5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 56 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
