WB result of p16 ARC Recombinant Rabbit mAb
Primary antibody: p16 ARC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
ARPC5/p16 ARC Recombinant Rabbit mAb (S-3564-23)
ARPC5/p16 ARC Recombinant Rabbit mAb (S-3564-23)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ARPC5/p16 |
| Synonyms | Actin-related protein 2/3 complex subunit 5; Arp2/3 complex 16 kDa subunit (p16-ARC); ARC16 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell projection |
| Accession | O15511 |
| Clone Number | S-3564-23 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | THP-1, HeLa, A549, PANC-1, PC-3, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
p16 ARC serves as a substrate for MAPK-activated protein kinase 2 (MAPKAPK2), which phosphorylates the protein at serine-77, potentially modulating Arp2/3 complex regulatory functions and actin cytoskeleton remodeling. As an essential component for maintaining Arp2/3 complex integrity, p16 ARC is critical for effective actin nucleation and cellular processes involving cytoskeletal dynamics.
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Western Blot
WB result of p16 ARC Recombinant Rabbit mAb
Primary antibody: p16 ARC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
WB result of p16 ARC Recombinant Rabbit mAb
Primary antibody: p16 ARC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
WB result of p16 ARC Recombinant Rabbit mAb
Primary antibody: p16 ARC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
