WB result of AQR/IBP160 Recombinant Rabbit mAb
Primary antibody: AQR/IBP160 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 171 kDa
Observed MW: 180 kDa
AQR/IBP160 Recombinant Rabbit mAb (S-1329-248)
AQR/IBP160 Recombinant Rabbit mAb (S-1329-248)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AQR/IBP160 |
| Synonyms | RNA helicase aquarius; Intron-binding protein of 160 kDa (IBP160); KIAA0560 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O60306 |
| Clone Number | S-1329-248 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, HEK-293, HepG2 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
AQR/IBP160 protein, also known as Aquarius homolog or Intron-binding protein of 160 kDa, is a spliceosomal intron-binding protein involved in pre-mRNA splicing and small nucleolar ribonucleoprotein (snoRNP) biogenesis. It links the position-dependent assembly of intron-encoded box C/D snoRNPs to pre-mRNA splicing, playing a key role in the folding of snoRNA sequences. This protein has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang. It binds to introns of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns during the late stages of splicing.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti- AQR/IBP160 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
