WB result of Anterior Gradient 2 Rabbit pAb
Primary antibody: Anterior Gradient 2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Lane 5: COLO 205 whole cell lysate 20 µg
Lane 6: A549 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
Anterior Gradient 2 Rabbit Polyclonal Antibody
Anterior Gradient 2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Anterior Gradient 2 |
| Synonyms | Anterior gradient protein 2 homolog; AG-2; hAG-2; HPC8; Secreted cement gland protein XAG-2 homolog; AG2; AGR2 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | O95994 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HT-29, MCF7, Caco-2, COLO 205, A549, mouse stomach, rat stomach |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Anterior Gradient 2 (AGR2) is an ~20 kDa endoplasmic-reticulum–resident protein disulfide-isomerase that catalyzes disulfide-bond formation in nascent mucins and other secreted proteins, acts as a molecular chaperone to maintain ER proteostasis, and is highly expressed in mucosal epithelia; in cancers its overexpression is linked to aggressive growth, metastasis, chemoresistance and suppression of type-I interferon responses, while its loss triggers ER stress and the unfolded-protein response, and a secreted monomeric form detectable in serum and urine serves as a promising extracellular biomarker.
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Western Blot
WB result of Anterior Gradient 2 Rabbit pAb
Primary antibody: Anterior Gradient 2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
WB result of Anterior Gradient 2 Rabbit pAb
Primary antibody: Anterior Gradient 2 Rabbit pAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Anterior Gradient 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
