WB result of ALOX5 Recombinant Rabbit mAb
Primary antibody: ALOX5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa
ALOX5 Recombinant Rabbit mAb (S-1980-27)
ALOX5 Recombinant Rabbit mAb (S-1980-27)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ALOX5 |
| Synonyms | Polyunsaturated fatty acid 5-lipoxygenase; Arachidonate 5-lipoxygenase (5-LO; 5-lipoxygenase); LOG5 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P09917 |
| Clone Number | S-1980-27 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | A431, Raji |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
ALOX5, also known as 5-lipoxygenase, is a key enzyme involved in the metabolism of arachidonic acid, a polyunsaturated fatty acid. It catalyzes the conversion of arachidonic acid to leukotriene A4, which is then further metabolized into various leukotrienes such as leukotriene B4 and cysteinyl leukotrienes. These leukotrienes play significant roles in inflammatory and allergic responses, including bronchoconstriction, increased vascular permeability, and chemotaxis of immune cells. ALOX5 is primarily expressed in immune cells like neutrophils, macrophages, and mast cells. Its activity is tightly regulated at multiple levels, including transcriptional control, post-translational modifications, and interaction with other proteins. Inhibitors of ALOX5 have been developed as potential therapeutic agents for conditions like asthma, allergic rhinitis, and certain inflammatory diseases, aiming to reduce the production of pro-inflammatory leukotrienes and alleviate associated symptoms.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
