WB result of Akt2 Recombinant Rabbit mAb
Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: JAR whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
AKT2 Recombinant Rabbit mAb (S-3256)
AKT2 Recombinant Rabbit mAb (S-3256)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AKT2 |
| Synonyms | RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta (PKB beta); RAC protein kinase beta (RAC-PK-beta) |
| Location | Cytoplasm, Nucleus |
| Accession | P31751 |
| Clone Number | S-3256 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | MEF, Jurkat, JAR, HeLa, NIH/3T3, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
AKT2 (also known as Protein Kinase B β, PKBβ) is a 481-residue serine/threonine kinase ubiquitously expressed but enriched in insulin-responsive tissues, where it transduces signals from the PI3K pathway through dual phosphorylation at T309 by PDK1 and S474 by mTORC2 to adopt an active conformation that phosphorylates >100 substrates—key among them GSK3β, FOXO1, AS160, and mTOR—thereby orchestrating glucose uptake via GLUT4 translocation, glycogen synthesis, cell survival, proliferation, and lipid metabolism; its dysregulation through activating E17K mutations, amplifications, or loss of PTEN contributes to type 2 diabetes, metabolic syndrome, and multiple cancers, making AKT2 a pivotal therapeutic target for isoform-selective inhibitors and metabolic modulators.
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Western Blot
WB result of Akt2 Recombinant Rabbit mAb
Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
WB result of Akt2 Recombinant Rabbit mAb
Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
WB result of Akt2 Recombinant Rabbit mAb
Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
