Immobilized Human Haptoglobin Protein, His tag at 0.2 μg/mL (50 μL/well) can bind Haptoglobin Recombinant Rabbit mAb (SDT-339-43) (Cat. No. S0B3126) with EC50 of 3.422-4.259 ng/mL.
Human Haptoglobin Protein, His tag
Human Haptoglobin Protein, His tag
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$320 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Zonulin, HP |
| Accession | P00738 |
| Amino Acid Sequence |
Protein sequence1 (P00738, Val19-Gln160, with C-His tag) VDSGNDVTDIADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNDKKQWINKAVGDKLPECEADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNNEKQWINKAVGDKLPECEAVCGKPKNPANPVQ; Protein sequence2 (P00738, Ile162-Asn406, with C-His tag) ILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN |
| Expression System | HEK293 |
| Molecular Weight | Predicted MW: 17.6 kDa(alpha chain)&29.0 kDa(beta chain) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | with C-His tag (alpha chain)&C-His tag (beta chain) |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
Haptoglobin (abbreviated as Hp) is the protein that in humans is encoded by the HP gene. In blood plasma, haptoglobin binds with high affinity to free hemoglobin released from erythrocytes, and thereby inhibits its deleterious oxidative activity. Compared to Hp, hemopexin binds to free heme. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). Haptoglobin is an acute-phase protein, its concentration in plasma changes in pathology, and the test for its concentration is part of normal clinical practice. Haptoglobin is a conservative protein synthesized mainly in the liver and lungs and is the subject of research as a potential biomarker of many diseases, including various forms of malignant neoplasms.
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Bioactivity
SDS-PAGE
2 μg（R: reducing conditions）
