Mouse Single-Cell BCR FL Amplification Kit (BD Platform)
Mouse Single-Cell BCR FL Amplification Kit (BD Platform)
Product Details
Product Details
Product Specification
Background
This product is mainly used for the determination of the paired full-length VDJ sequences in mouse-derived single B cells. This product is compatible with the BD Rhapsody single-cell sequencing platform. It adopts optimized target amplification primer sequences and the most suitable enzyme reaction system, which can effectively amplify and detect the light and heavy chain sequence information of mouse-derived single B cells, including IGHA, IGHG, IGHM, IGKC, IGLC, etc.
Components
Component |
Size |
Volume |
TSO |
1 |
30uL |
1M MgCl2 |
1 |
15uL |
Hybridization Buffer |
4 |
1.6mL |
Extension Enzyme |
1 |
70uL |
Extension Buffer |
1 |
160uL |
Extension Primers |
1 |
30uL |
dNTP |
1 |
200uL |
Nuclease Free Water |
2 |
1mL |
PCR MasterMix |
1 |
1.2mL |
Bead RT/PCR Enhancer |
1 |
200uL |
VDJ Universal Oligo N1 |
1 |
65uL |
VDJ Universal Oligo N2 |
1 |
30uL |
BCR N1 Primer |
1 |
16uL |
BCR N2 Primer |
1 |
40uL |
Elution Buffer |
2 |
1.8mL |
Protocol
Refer to the BD Rhapsody full-length VDJ library construction method.
File name: BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)
Document number: 23-24017(03)
Download link: https://scomix.bd.com/hc/en-us/articles/9285990045197-VDJ-full-length-TCR-BCR-assay-protocols-Human
Note: In this kit, the volume of Bead RT/PCR Enhancer is 40 µL, and the corresponding volume of Nuclease-Free Water is 45.2 µL.
