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Unione ®️ TR-FRET Human PD1/PD-L1 Binding Kit

Unione ®️ TR-FRET Human PD1/PD-L1 Binding Kit

Catalog Number: UA086026 Brand: UA BIOSCIENCE
Price:
Regular price $2,180 USD
Regular price Sale price $2,180 USD
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Product Details

Product Specification


Host Human
Stability & Storage

-80℃

Background

The assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between PD1 and PD-L1. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.

As shown in the figure, the interaction between PD1 and PD-L1 is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of PD1 and PD-L1 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Pembrolizumab, as a positive control, blocks the interaction between PD1 and PD-L1, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the PD1/PD-L1 interaction, the lower the signal. The specific signal is proportional to the extent of the PD1/PD-L1 interaction. This homogeneous assay is simple to perform and requires no washing steps.

Components

Component

Concentration

100T

500T

2500T

10000T

Storage Temperature

Pembrolizumab

5μM

5μL

10μL

50μL

200μL

-80℃

Tag1-PD-L1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Tag2- PD1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃


Note: Aliquot immediately upon first thaw and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.