Linearity
Three different mouse spleen cells concentrations, 8 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the three cell concentrations.
StepSlim Mouse IFN-γ (HRP) ELISpot Kit
StepSlim Mouse IFN-γ (HRP) ELISpot Kit
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$365 USD
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Product Details
Product Details
Product Specification
| Antibody Type | Recombinant mAb |
| Reactivity | Mouse |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8℃ as supplied. |
Kit
| Precision | Intra-assay: 5.77%； Inter-assay: 11.61% |
| Sample type | Spleen cells |
| Assay type | Sandwich (Qualitative) |
| Assay time | 18-24 hours |
| Species reactivity | Mouse |
| Plate | Non-Strip |
Background
Mouse IFN-γ, or Interferon-gamma, is a crucial cytokine primarily produced by natural killer (NK) cells and T lymphocytes, particularly Th1 cells and cytotoxic T cells. It plays a pivotal role in the immune response, acting as a key mediator in both innate and adaptive immunity. IFN-γ is known for its potent antiviral, immunoregulatory, and anti-tumor properties. It enhances the presentation of antigens by upregulating major histocompatibility complex (MHC) class I and II molecules on antigen-presenting cells, thereby facilitating a more robust immune response. Additionally, IFN-γ activates macrophages, increasing their ability to phagocytose and destroy pathogens. It also promotes the differentiation of naive T cells into Th1 cells, further amplifying the immune response. In research, mouse IFN-γ is widely used to study immune responses, inflammatory diseases, and host-pathogen interactions, providing valuable insights into the mechanisms of immune regulation and potential therapeutic strategies.
Picture
Picture
Elispot
Precision
Stimulate 2×10⁵ cells/well of mouse spleen cells with Con A for 20 hours at 37 °C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.
Diagram of spots
Stimulate 2×10⁵ cells/well of mouse spleen cells with Con A. After 20 hours of stimulation at 37 °C in a 5% CO₂ incubator, incubate with HRP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.
Protocol Diagram
