Linearity
Six different cryopreservation PBMCs concentrations, 4 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the six cell concentrations.
StepSlim Human IL-2 (ALP) ELISpot Kit
StepSlim Human IL-2 (ALP) ELISpot Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-2 |
| Antibody Type | Recombinant mAb |
| Reactivity | Human |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8℃ as supplied. |
Kit
| Precision | Intra-assay: 2.44%； Inter-assay: 3.51% |
| Sample type | PBMCs |
| Assay type | Sandwich (Qualitative) |
| Assay time | 18-24hours |
| Species reactivity | Human |
| Plate | Non-Strip |
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self-antigens", which could result in "autoimmunity". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
Picture
Picture
Elispot
Precision
Stimulate 2×10⁵ cells/well of cryopreservation PBMCs with PHA for 22 hours at 37°C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.
Diagram of spots
Stimulate 2×10⁵ cells/well of cryopreserved PBMCs with PHA. After 22 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with ALP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.
Protocol Diagram
