Linearity
Six different cryopreservation PBMCs concentrations, 4 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the six cell concentrations
StepSlim Human IFN-γ（ALP）plus ELISpot Kit
StepSlim Human IFN-γ（ALP）plus ELISpot Kit
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$400 USD
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Product Details
Product Details
Product Specification
| Antigen | IFN-γ |
| Antibody Type | Recombinant mAb |
| Reactivity | Human |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8℃ as supplied. |
Kit
| Precision | Intra-assay: 2.08%； Inter-assay: 2.44% |
| Sample type | PBMCs |
| Assay type | Sandwich (Qualitative) |
| Assay time | 18-24hours |
| Species reactivity | Human |
Background
IFN-γ
Picture
Picture
Elispot
Precision
Stimulate 1×10⁵ cells/well of cryopreservation PBMCs with PHA for 20 hours at 37°C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation
Stimulate 1×10⁵ cells/well of cryopreservation PBMCs with PHA for 20 hours at 37°C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation
Diagram of spots
Stimulate 1×10⁵ cells/well of cryopreserved PBMCs with PHA. After 20 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with ALP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.
Protocol Diagram
Competitor Comparison
Stimulate 1×10⁵ cells/well of cryopreserved PBMCs with PHA, 4 replicates, 1 batch. After 20 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with ALP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.
Stimulate 1×10⁵ cells/well of cryopreserved PBMCs with PHA, 4 replicates, 1 batch. After 20 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with ALP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.
