• Monkey IL-6 ELISA Kit
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Monkey IL-6 ELISA Kit

Monkey IL-6 ELISA Kit

Catalog Number: abs554110 Application: ELISA Reactivity: Monkey Conjugation:
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$429 USD
$429 USD
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Product Details

Product Specification

protein IL-6
Species Reactivity Simian
Theory This kit adopts the principle of sandwich method. The specific anti-monkey IL-6 antibody was coated in a 96-well microplate, and monkey IL-6 standard or sample was added to the microwells, so that monkey IL-6 protein in the standard or monkey IL-6 protein in the sample was bound to the anti-monkey IL-6 antibody solid on the microplate, then biotinylated anti-monkey IL-6 antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the monkey IL-6 protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.
Source Monkey
Synonym IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte
Detection Type Recombinant or native monkey IL-6 can be detected and does not cross-react with other related proteins
Composition
Chinese Name 96T Preservation conditions
Enzyme labeled plate (detachable) 12 Strip x 8 Hole 4°C/-20°C
Lyophilized Standard 2 4°C/-20°C
Standard & Sample dilution 20 mL 4°C/-20°C
Concentrated biotinylated antibodies ( 100× ） 120 μL 4°C/-20°C
Biotinylated antibody dilution 12 mL 4°C/-20°C
concentrate HRP Enzyme conjugate ( 100× ） 120 μL 4°C/-20°C
Enzyme conjugate dilution 12 mL 4°C/-20°C
Concentrated wash ( 25× ） 20 mL 4°C/-20°C
Chromogenic substrate solution ( TMB ） 10 mL 4°C/-20°C( Protected from light)
Reaction stop solution 6 mL 4°C/-20°C
Sealing film 2 normal temperature
General Notes

1. If the entire kit is stored at-20 °C, please place the kit at 4 °C the night before the experiment.

2. Salt precipitation will occur when the concentrated washing liquid is stored at low temperature. When diluted, it can be heated in a water bath to help dissolve.

3. The newly opened wells of the enzyme labeled plate may contain a little water-like substance, which is a normal phenomenon and will not have any impact on the experimental results.

4. This kit is intended for laboratory research and development use only, not for human or animal use.

5. Reagents should be treated as hazardous substances and should be handled carefully and properly disposed of.

6. Gloves, lab coats and protective glasses should always be worn to avoid skin and eye contact with terminating solution and TMB. In case of accidental contact, please wash thoroughly with water.
Storage Temp. Unopened kit, stored at 4 °C, shelf life 6 months.
Test Range 15.63-1000 pg/mL; Sensitivity: 5.8 pg/mL