Protein A Donor Beads

The Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer luminescence between donor beads and acceptor beads.

Donor beads recognize Protein 1 (Tag1 label), while Acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the distance between the beads becomes less than 200nm. Upon excitation at 680nm, the Donor beads generate singlet oxygen, which diffuses to the Acceptor beads. The Acceptor beads then undergo a redox reaction, emitting light at 615nm. The signal intensity is directly proportional to the strength of protein interaction.

This product features a simple operation process, requires no washing, offers fast results, and high sensitivity. It is capable of detecting weak interactions.

1. This experiment is light-sensitive. Avoid light exposure during operation. It is recommended to perform preparation, sample loading, and incubation steps under green light (illuminance below 100 LUX).

2. This product is compatible with microplate readers equipped with Alpha detection modules.

3. Vortex thoroughly before use, or briefly centrifuge (2000×g, 5–10 seconds) to ensure complete usage.

4. It is recommended to use the dilution buffer provided by our company for reagent preparation and sample dilution. If additional components are required, they can be directly added to this buffer.

5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and time.

6. Avoid generating bubbles during sample loading.