One-step rapid mycoplasma detection kit (isothermal amplification)
One-step rapid mycoplasma detection kit (isothermal amplification)
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Product Specification
| Usage |
I. Preparation before the experiment: 65°C water bath or metal bath II. Operation steps: 1. Preparation of samples to be tested (1) Adherent cells: Directly aspirate the supernatant. It is recommended to take samples when the cells are inoculated or the medium is changed for more than 3 days and the confluence reaches about 90%. At this time, the mycoplasma content in the supernatant is high and easy to detect. (2) Suspended cells: Centrifuge at 1000 rpm for 5 minutes and then take the supernatant. It is recommended to take samples when the cells are inoculated or the medium is changed for more than 3 days. At this time, the mycoplasma content in the cell culture medium is high and easy to detect. 2. Reaction system
(2) After preparing the above reaction system according to the number of samples, divide it into reaction tubes, add 20 uL of Paraffin Oil, and then proceed to the next step. 3. Sample addition (1) Negative control: do not add any sample to the first reaction tube as a negative control; (2) Positive control: add 1 uL of Positive Control to the last reaction tube as a positive control; (3) Sample to be tested: add 1 uL of cell culture supernatant to the remaining reaction tubes. Please extend the pipette tip below the surface of Paraffin Oil to add the sample. 4. Reaction conditions. Transfer the reaction tube to a preheated 65°C water bath or metal bath and incubate for 30 minutes. III. Result determination After the reaction is completed, remove the reaction tube immediately and wait for it to return to room temperature. If the reaction solution is still yellow, the result is negative; if the reaction solution turns red, the result is positive. The reaction tube must not be opened, otherwise it will easily lead to aerosol contamination. |
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| Description | This product utilizes isothermal amplification technology and a visual color change to rapidly detect eight common mycoplasma species in cell culture fluids. No DNA extraction is required; 1 μL of cell culture supernatant is added directly to the reaction solution and the reaction is incubated at 65°C for 30 minutes. The color of the reaction solution changes from yellow to red for positive samples. The entire process does not require a PCR instrument or electrophoresis, effectively preventing cross-contamination caused by aerosols. Compared to traditional PCR methods, this product offers ease of use and exceptional sensitivity. It is tolerant to various PCR inhibitors in the culture medium, preventing false negatives and demonstrating high consistency with qPCR results. Product composition:
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| General Notes | 1. Mix Mycored Buffer thoroughly before use. 2. Do not open the reaction tube after the reaction is complete. Isothermal amplification produces a large amount of product, and aerosols and other factors can easily contaminate the experimental environment. 3. It is recommended to use filter tips when preparing the reaction solution. Discarded tips and reaction tubes in a ziplock bag for prompt disposal. 4. To ensure the reliability and stability of cell experiments, it is recommended to perform regular mycoplasma contamination testing. 5. For your safety and health, please wear a lab coat and disposable gloves during operation. |
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| Storage Temp. | Store at -20℃, away from light, valid for 12 months. |
