Standard curve
Example of Mouse C3 standard curve in Assay Diluent #1.
Mouse C3 OneStep ELISA Kit
Mouse C3 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | C3 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.0%； Inter-assay: 7.2% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 8.854 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Recovery | Cell culture supernatant: 86% Serum: 90% Plasma: 116% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse C3 is a central and essential component of the complement system, acting as the pivotal convergence point for all three complement activation pathways: classical, lectin, and alternative. This ~186 kDa protein, primarily synthesized in the liver, is proteolytically cleaved into two key fragments upon activation: the anaphylatoxin C3a, which recruits and activates immune cells, and the opsonin C3b, which tags pathogens for phagocytosis and amplifies the complement cascade. Its critical roles include mediating innate immune defense against infections, clearing immune complexes and apoptotic cells, and bridging innate and adaptive immunity. Consequently, Mouse C3 is a major focus of research in infectious disease, autoimmunity (e.g., lupus), neuroinflammation, and ischemia-reperfusion injury, with genetically modified C3-deficient mice being a fundamental tool for elucidating its precise functions in vivo.
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ELISA
Linearity
The concentrations of Mouse C3 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: C57 Mouse serum was 3×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 184.73 ug/mL in C57 Mouse serum.
#2 sample is undiluted samples are as follows: C57 Mouse plasma-EDTA was 3×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 138.74 ug/mL in C57 Mouse plasma-EDTA.
#3 sample is undiluted samples are as follows: C57 Mouse plasma -Heparin was 3×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 138.92 ug/mL in C57 Mouse plasma -Heparin.
#4 sample is undiluted samples are as follows: C57 Mouse plasma -Citrate was 3×10^5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 181.21 ug/mL in C57 Mouse plasma -Citrate.
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Protocol Diagram
