Standard curve
Example of Monkey GDF-15 standard curve in Assay Diluent #12YS2.
Monkey GDF-15(serum/plasma) OneStep ELISA Kit
Monkey GDF-15(serum/plasma) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | GDF-15 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.3%； Inter-assay: 4.1% |
| Sample type | Serum,Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.201 pg/mL |
| Range | 7.81 pg/mL –500 pg/mL |
| Recovery | Serum: 109% Plasma: 112% |
| Assay time | 60 minutes |
| Species reactivity | Mk |
Picture
Picture
ELISA
Linearity
The concentrations of Monkey GDF-15 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: Monkey serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1187.16pg/mL in Monkey serum.
To assess the linearity of the assay, two samples were spiked with high concentrations of Monkey GDF-15 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
