Standard curve
Example of Human IL-7 standard curve in Assay Diluent #11Y.
Human IL-7 OneStep ELISA Kit
Human IL-7 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-7 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.7%； Inter-assay: 6.0% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.653 pg/ml |
| Range | 1.56 pg/mL – 100 pg/mL |
| Recovery | Cell culture supernatant: 115% Serum: 98% Plasma: 114% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide. Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Like other common gamma-chain cytokines like IL-2 and IL-15, IL-7 and its receptor, IL-7R, have been used in a variety of immunotherapy applications, often in fluid tumors and in some instances of solid tumor models.
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ELISA
Linearity
The concentrations of Human IL-7 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human Serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 11.90 pg/mL in Human Serum.
#2 sample is undiluted samples are as follows: Human Plasma was 4 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.19 pg/mL in Human Plasma.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-7 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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