Standard curve
Example of Human IL-1β standard curve in Assay Diluent #12E5.
Human IL-1β OneStep ELISA Kit (High Level)
Human IL-1β OneStep ELISA Kit (High Level)
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Product Details
Product Details
Product Specification
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 5.0%； Inter-assay: 6.5% |
| Sample type | Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.335 pg/ml |
| Range | 7.8 pg/mL – 500 pg/mL |
| Recovery | Serum: 105% Plasma: 83% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Picture
Picture
ELISA
Linearity
The concentrations of Human IL-1β were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 942.47 pg/mL in stimulated human PBMC supernatant.
Protocol Diagram
