Standard curve
Example of Human CCL17 standard curve in Assay Diluent #10.
Human CCL17 OneStep ELISA Kit
Human CCL17 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | CCL17 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.0%； Inter-assay: 5.0% |
| Sample type | Cell culture supernatant, Serum,Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 4.883 pg/ml |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Recovery | Cell culture supernatant: 98% Serum: 100% Plasma: 91% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
CCL17 is a powerful chemokine produced in the thymus and by antigen-presenting cells like dendritic cells, macrophages, and monocytes. CCL17 plays a complex role in cancer. It attracts T-regulatory cells allowing for some cancers to evade an immune response. However, in other cancers, such as melanoma, an increase in CCL17 is linked to an improved outcome. CCL17 has also been linked to autoimmune and allergic diseases. CCL17 as well as its partner chemokine CCL22 induce chemotaxis in T-helper cells. They do this by binding to CCR4. CCL17 was the first CC chemokine identified that interacted with T cells with high affinity. CCL17 was also found to interact with monocytes, but with less affinity. It does not interact with granulocytes.
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Picture
ELISA
Linearity
The concentrations of Human CCL17 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 3days (30%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.82 ng/mL in stimulated human PBMC supernatant.
Linearity
The concentrations of Human CCL17 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human Serum was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 124.5 pg/mL in Human Serum.
#2 sample is undiluted samples are as follows: Human Plasma was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 81.91pg/mL in Human Plasma.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human CCL17 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
