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Chikungunya virus (CHIKV) (strain SL-CK1) E2 OneStep ELISA Kit

Chikungunya virus (CHIKV) (strain SL-CK1) E2 OneStep ELISA Kit

Catalog Number: S0C3098 Reactivity: Other Conjugation: Brand: Starter
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Regular price $698.00 SGD
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Product Details

Product Specification


Antigen Chikungunya virus (CHIKV) (strain SL-CK1) E2
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Others
Purification Protein A
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

Kit


Precision Intra-assay: 3.9%;
Inter-assay: 4.9%
Sample type Cell culture supernatant; Serum; Plasma
Assay type Sandwich (quantitative)
Sensitivity 14.713 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Recovery Cell culture supernatant: 100%
Serum: 101%
Plasma: 106%
Assay time 60 minutes
Species reactivity Others

Background

The CHIKV E2 protein is a critical structural glycoprotein on the surface of the Chikungunya virus. It is primarily responsible for attaching to and entering host cells by binding to specific receptors. As the most exposed part of the virus, E2 serves as the main target for neutralizing antibodies. This makes it a key antigen for immune recognition and response. Consequently, it is a major focus in the development of vaccines and diagnostic tests. Research into E2 is essential for designing effective therapeutics against CHIKV infections. Its functional importance also extends to mediating viral fusion alongside the E1 protein. Overall, E2 plays a central role in both viral infectivity and protective immunity.

Picture

ELISA

Standard curve
Example of CHIKV E2 standard curve in Assay Diluent #1.

Standard curve
Example of CHIKV E2 standard curve in Assay Diluent #11.

To assess the linearity of the assay, three samples were spiked with high concentrations of CHIKV E2 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Protocol Diagram