IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Hepatocyte Specific Antigen |
| Synonyms | HSA; Hep Par-1; OCH1E5 |
| Location | Cytoplasm |
| Clone Number | SDT-4389 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | / |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:2000 | Hu |
Background
Hepatocyte Specific Antigen (HSA), also known as Hep Par-1 or liver-enriched transcription factor, is a mitochondrially localized protein uniquely expressed in hepatocytes that serves as a highly specific immunohistochemical marker for liver-derived tissues and hepatocellular carcinomas, playing critical roles in hepatic differentiation, metabolic regulation, and maintaining liver-specific functions through its involvement in mitochondrial energy metabolism and urea cycle processes.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human cervical cancer. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat lung. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression Atlas
Expression of hepatocyte specific antigen in tumor tissues.
Expression of hepatocyte specific antigen in human tissues.
Expression of hepatocyte specific antigen in mouse & rat tissues.
