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Hepatocyte Specific Antigen Recombinant Mouse mAb (SDT-4389)

Hepatocyte Specific Antigen Recombinant Mouse mAb (SDT-4389)

Catalog Number: S0B2473 Application: IHC-P Reactivity: Human,Mouse, Rat Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen Hepatocyte Specific Antigen
Synonyms HSA; Hep Par-1; OCH1E5
Location Cytoplasm
Clone Number SDT-4389
Antibody Type Mouse mAb
Isotype IgG1
Application IHC-P
Reactivity Hu, Ms, Rt
Predicted Reactivity /
Purification Protein G
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
IHC-P 1:2000 Hu

Background

Hepatocyte Specific Antigen (HSA), also known as Hep Par-1 or liver-enriched transcription factor, is a mitochondrially localized protein uniquely expressed in hepatocytes that serves as a highly specific immunohistochemical marker for liver-derived tissues and hepatocellular carcinomas, playing critical roles in hepatic differentiation, metabolic regulation, and maintaining liver-specific functions through its involvement in mitochondrial energy metabolism and urea cycle processes.

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Immunohistochemistry

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cervical cancer. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat lung. Anti-Hepatocyte specific antigen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Expression Atlas

Expression of hepatocyte specific antigen in tumor tissues.

Expression of hepatocyte specific antigen in human tissues.

Expression of hepatocyte specific antigen in mouse & rat tissues.