Flow cytometric analysis of Human CD103 expression on Human PBMC. Human PBMC (peripheral blood mononuclear cells) were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Percp-Cy5.5 Mouse IgG1, κ Isotype Control (Left panel) or Percp-Cy5.5 Mouse Anti-Human CD103 Antibody (Right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Mouse Anti-Human CD103 Antibody (S-2876)
PerCP-Cy5.5 Mouse Anti-Human CD103 Antibody (S-2876)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD103 |
| Synonyms | Integrin alpha-E; HML-1 antigen; Integrin alpha-IEL; Mucosal lymphocyte 1 antigen; ITGAE |
| Location | Membrane |
| Accession | P38570 |
| Clone Number | S-2876 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD103, also known as integrin αEβ7, is a transmembrane heterodimer complex primarily expressed on tissue-resident memory T cells (T RM cells), regulatory T cells (Tregs), and certain dendritic cells. It binds to E-cadherin on epithelial cells, mediating lymphocyte adhesion and retention in tissues such as the skin, gut, and lungs. This interaction is crucial for immune surveillance and maintaining tissue integrity. CD103 expression is regulated by TGF-β, which enhances its ability to bind E-cadherin. In cancer research, CD103 is significant in tumor-infiltrating lymphocytes (TILs), influencing immune responses within tumors. Additionally, CD103 plays a role in cytotoxic T cell activation and effective tumor cell lysis.
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