Flow cytometric analysis of Human CD235a/b expression on human peripheral blood. human peripheral blood cells were stained with V500 Mouse Anti-Human CD45 Antibody and either PE Mouse IgG2b, κ Isotype Control (Left panel) or SDT PE Mouse Anti-Human CD235a/b Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE Mouse Anti-Human CD235a/b Antibody (S-R403)
PE Mouse Anti-Human CD235a/b Antibody (S-R403)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD235a/b |
| Synonyms | Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GYPA, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, GYPB |
| Location | Cell membrane |
| Accession | P02724、P06028 |
| Clone Number | S-R403 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
Glycophorin A (GYPA) and B (GYPB; this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include the Miltenberger (Mi) complex and several isoforms of Stones (Sta); also Dantu, Sat, Henshaw (He or MNS6), Mg and deletion variants Ena, S-s-U- and Mk. Most of these are the result of gene recombinations between GYPA and GYPB.
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