Flow cytometric analysis of Human CD138 expression on U266B1 cells. Cells from the U266B1 (Human multiple myeloma B lymphocyte, Right) or Jurkat (Human T cell leukemia T lymphocyte, Left) was stained with either Phycoerythrin Mouse IgG1 Isotype Control (Black line histogram) or SDT PE Mouse Anti-Human CD138 Antibody (Red line histogram) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
Host | Mouse |
Antigen | CD138 |
Synonyms | Syndecan-1; SYND1; SDC; SDC1 |
Location | Membrane |
Accession | P18827 |
Clone Number | S-R553 |
Antibody Type | Mouse mAb |
Isotype | IgG1 |
Application | FCM |
Reactivity | Hu |
Positive Sample | U266B1 |
Purification | Protein G |
Concentration | 0.2 mg/ml |
Conjugation | PE |
Physical Appearance | Liquid |
Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
application | dilution | species |
FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD138, also known as syndecan-1, is a transmembrane heparan sulfate proteoglycan that plays a crucial role in cell adhesion, migration, and signaling. It is predominantly expressed on the surface of plasma cells and certain epithelial cells. In plasma cells, CD138 serves as a valuable marker for their identification and isolation, aiding in the diagnosis and study of plasma cell disorders such as multiple myomael. On epithelial cells, it contributes to the maintenance of cell structure and function by interacting with extracellular matrix components and growth factors. Additionally, CD138 is involved in various biological processes including wound healing, inflammation, and cancer progression. Its ability to bind and modulate the activity of numerous proteins makes it an important factor in cellular communication and regulation.
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