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Pacific Blue Rat Anti-Mouse CD16/CD32 Antibody (S-R368)

Pacific Blue Rat Anti-Mouse CD16/CD32 Antibody (S-R368)

Catalog Number: S0B8279 Application: FCM Reactivity: Mouse Conjugation: Pacific Blue Brand: Starter
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$111.00 SGD
$111.00 SGD
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Product Details

Product Specification


Host Rat
Antigen CD16/CD32
Synonyms Low affinity immunoglobulin gamma Fc region receptor III; IgG Fc receptor III; Fc-gamma RIII (FcRIII); Fcgr3; Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII; IgG Fc receptor II beta; Lymphocyte antigen 17 (Ly-17); Fcgr2; Fcgr2b; Ly-17
Location Cell membrane
Accession P08508、P08101
Clone Number S-R368
Antibody Type Rat mAb
Isotype IgG2b,k
Application FCM
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Pacific Blue
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

CD16/CD32 proteins, also known as FcγRIII and FcγRII, are cell surface receptors expressed on immune cells such as natural killer cells, macrophages, and certain T cell subsets; they bind the Fc region of immunoglobulin G to mediate antibody-dependent cellular cytotoxicity, phagocytosis, and immune complex clearance by triggering activating (ITAM-based) or inhibitory (ITIM-based) signaling cascades that modulate innate and adaptive immune responses.

Picture

FC

Flow cytometric analysis of CD16/CD32 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with either Pacific Blue Rat IgG2b, κ Isotype Control (Black line histogram) or SDT Pacific Blue Rat Anti-Mouse CD16/CD32 antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.