Flow cytometric analysis of Wistar Rat splenocytes labelling CD3 antibody at 1/200 (1 μg) dilution (Right panel) compared with a Mouse IgG2b Isotype Control (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - PE antibody separately.
Mouse Anti-Rat CD3 Antibody (S-3399)
Mouse Anti-Rat CD3 Antibody (S-3399)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD3 |
| Synonyms | T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; Cd3g |
| Location | Cell membrane |
| Accession | Q64159、P19377 |
| Clone Number | S-3399 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | Wistar Rat splenocytes |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Rt |
Background
CD3 is a multimeric protein complex that serves as a crucial T-cell co-receptor, composed of four distinct chains (CD3γ, δ, ε, ζ) that non-covalently associate with the T-cell receptor (TCR), where its cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) become phosphorylated upon antigen recognition to initiate downstream signaling cascades leading to T-cell activation, differentiation, and effector functions, making it an essential component of adaptive immunity and a valuable target for therapeutic interventions such as the immunosuppressive agent muromonab-CD3 and emerging bispecific antibodies in oncology.
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