Flow cytometric analysis of Foxp3 / Transcription Factor Staining Buffer Set fixed and permeabilized Human PBMC (human peripheral blood mononuclear cells) labelling Perforin antibody at 1/2000 dilution (0.1 μg) / (Right panel) compared with a Mouse IgG2b, κ (Left panel) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8 – Percp/Cy5.5 antibody separately.
Mouse Anti-Human Perforin Antibody (S-3064)
Mouse Anti-Human Perforin Antibody (S-3064)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Perforin |
| Synonyms | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein (PFP); PFP; PRF1 |
| Location | Secreted, Cell membrane |
| Accession | P14222 |
| Clone Number | S-3064 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:2000 | Hu |
Background
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions as a pore-forming protein, inserting itself into the membranes of target cells, such as virus-infected or cancerous cells. Once integrated, perforin creates pores that disrupt the cell membrane's integrity, leading to osmotic lysis and ultimately killing the target cell. This mechanism is essential for the immune system's ability to eliminate harmful cells and maintain homeostasis.
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