1 1

FMC63 Recombinant Rabbit mAb (APC Conjugate) (S-2163-77)

FMC63 Recombinant Rabbit mAb (APC Conjugate) (S-2163-77)

Catalog Number: S0B8214 Application: FCM Brand: Starter
Share:
Price:
$1,305.00 SGD
$1,305.00 SGD
Size:
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Immunogen Recombinant Protein
Clone Number S-2163-77
Antibody Type Recombinant mAb
Isotype IgG
Application FCM
Purification Protein A
Concentration 0.2 mg/ml
Conjugation APC
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Species independent

Background

FMC63 scFv (single-chain variable fragment) is a recombinant protein derived from the variable regions (VH and VL) of the anti-CD19 monoclonal antibody FMC63, linked by a flexible peptide spacer (e.g., (Gly₄Ser)₃). It specifically binds to the B-cell surface antigen CD19 with high affinity (KD ~1-10 nM) and is a key targeting component in FDA-approved CAR-T therapies, such as Kymriah®. The humanized FMC63 scFv retains potent antigen recognition while minimizing immunogenicity, making it highly effective in treating B-cell malignancies, including acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Ongoing research focuses on optimizing its affinity, stability, and functionality to enhance the safety and efficacy of CAR-T cell therapies.

Picture

FC

Flow cytometric analysis of FMC63 expression on CAR (FMC63 scFv) transfected 293T (Human embryonic kidney epithelial cell. CAR (FMC63 scFv) transfected 293T were stained with APC Rabbit IgG Isotype Control (Left panel) or SDT APC Rabbit Anti-FMC63 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.