Flow cytometric analysis of Perforin expression in PBMC (Human peripheral blood mononuclear cells). Human PBMC were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with Percp/Cy5.5 Mouse Anti-Human CD8 Antibody and either Mouse IgG2b, κ Isotype Control or SDT FITC Mouse Anti-Human Perforin Antibody at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human Perforin Antibody (S-3064)
FITC Mouse Anti-Human Perforin Antibody (S-3064)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Perforin |
| Synonyms | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein (PFP); PFP; PRF1 |
| Location | Secreted, Cell membrane |
| Accession | P14222 |
| Clone Number | S-3064 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1.25μl per million cells in 100μl volume | Hu |
Background
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions as a pore-forming protein, inserting itself into the membranes of target cells, such as virus-infected or cancerous cells. Once integrated, perforin creates pores that disrupt the cell membrane's integrity, leading to osmotic lysis and ultimately killing the target cell. This mechanism is essential for the immune system's ability to eliminate harmful cells and maintain homeostasis.
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