Flow cytometric analysis of Human CD185 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 Antibody and either Biotin Mouse IgG1, κ Isotype Control (Left panel) or SDT Biotin Mouse Anti-Human CD185 Antibody (Right panel) at 5μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human CD185 Antibody (S-R653)
Biotin Mouse Anti-Human CD185 Antibody (S-R653)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD185 |
| Synonyms | C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; Burkitt lymphoma receptor 1; Monocyte-derived receptor 15 (MDR-15); BLR1; MDR15; CXCR5 |
| Location | Cell membrane |
| Accession | P32302 |
| Clone Number | S-R653 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD185, also known as CXCR5, is a 43 kDa G protein-coupled chemokine receptor expressed on follicular helper T (Tfh) cells, B cells, and some memory T cells, where it guides these immune cells into B-cell follicles within secondary lymphoid organs by binding its ligand CXCL13, thereby orchestrating germinal-center formation, affinity maturation and class-switching of antibodies; dysregulated CXCR5 signaling is implicated in autoimmune diseases such as systemic lupus erythematosus and in certain lymphomas, making it a potential therapeutic target.
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