Flow cytometric analysis of Human CD133 expression on Human PBMC. Human PBMC (peripheral blood mononuclear cells) were stained with either Mouse IgG1, κ Isotype Control (Left panel) or APC Mouse Anti-Human CD133 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC Mouse Anti-Human CD133 Antibody (S-R586)
APC Mouse Anti-Human CD133 Antibody (S-R586)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD133 |
| Synonyms | Prominin-1; Antigen AC133; Prominin-like protein 1; PROML1; PROM1 |
| Location | Endoplasmic reticulum, Apical cell membrane |
| Accession | O43490 |
| Clone Number | S-R586 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD133, also known as prominin-1, is a pentaspan transmembrane glycoprotein encoded by the PROM1 gene. It is widely used as a biomarker for the isolation and identification of stem and progenitor cells in various normal tissues and cancer stem cells (CSCs) in different types of tumors. This 97-kDa protein has five transmembrane domains, with its N-terminus extending into the extracellular space and the C-terminus residing inside the cell. CD133 is expressed in neural progenitor cells, human hematopoietic cells, and various cancers. Its presence in cancer cells is often associated with poor prognosis, therapy resistance, and tumor metastasis.
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