Multiparameter flow cytometric analysis of human CD6 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD14 Antibody and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD6 Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD6 Antibody (S-688-33)
Alexa Fluor® 647 Mouse Anti-Human CD6 Antibody (S-688-33)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD6 |
| Synonyms | T-cell differentiation antigen CD6; T12; TP120 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P30203 |
| Clone Number | S-688-33 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD6 is a type I transmembrane glycoprotein with a molecular mass of 105–130 kDa. It is composed of an extracellular region with three tandem scavenger receptor cysteine-rich (SRCR) domains, a transmembrane region, and a cytoplasmic tail with several phosphorylatable residues suitable for intracellular signal transduction. CD6 associates with the TCR/CD3 complex and plays a role in stabilizing adhesive contacts with antigen-presenting cells (APCs), modulating subsequent TCR/CD3 intracellular signals that drive proliferative and differentiation responses. CD6 also interacts with several ligands, including CD166/ALCAM, CD318/CDCP1, and galectins 1 and 3. These interactions are important for various T cell physiological processes such as thymocyte development, T-APC immunological synapse stabilization, and T cell transmigration to inflamed tissues. Moreover, CD6 has been implicated in various autoimmune diseases. It was identified as a susceptibility locus in multiple sclerosis and Behcet's disease. The role of CD6 in autoimmune diseases may specifically reflect its interaction with CD318, which is expressed on key tissue cells in these diseases.
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