Standard curve
Example of Rat IL-2 standard curve in Assay Diluent #12S2.
Rat IL-2 (serum + plasma) OneStep ELISA Kit
Rat IL-2 (serum + plasma) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-2 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rt |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.1%； Inter-assay: 4.2% |
| Sample type | Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 17.83 pg/mL |
| Range | 93.75 pg/mL – 6000 pg/mL |
| Recovery | Serum: 109% Plasma: 111% |
| Assay time | 60 minutes |
| Species reactivity | Rt |
Background
Rat IL-2 (Interleukin-2) is a 15–17 kDa glycoprotein and key T-cell growth factor primarily secreted by activated CD4+ T cells, NK cells, and dendritic cells. It drives T-cell proliferation, Th1 differentiation, and NK cell activity while also supporting regulatory T cell (Treg) function for immune tolerance. Signaling through the IL-2 receptor (IL-2Rαβγ) activates JAK-STAT pathways, promoting cell survival and expansion. Rat IL-2 shares ~70% homology with mouse IL-2 but differs significantly from humanIL-2, requiring species-specific detection methods (e.g., ELISA, flow cytometry). It is crucial in research on autoimmunity (e.g., EAE), transplantation, and cancer immunotherapy, with dysregulation linked to immune disorders. Recombinant IL-2 has therapeutic potential but demands rat-specific reagents for accurate study.
Picture
Picture
ELISA
Linearity
Linearity
The concentrations of Rat IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: SHR Rat serum (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 239.11 pg/mL in SHR Rat serum.
S#1 sample is undiluted samples are as follows: Lewis Rat serum (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 191.36 pg/mL in Lewis Rat serum.
P#1 sample is undiluted samples are as follows: Lewis Rat plasma-Heparin (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 274.08 pg/mL in Lewis Rat plasma-Heparin.
Protocol Diagram
