Standard curve
Example of Rat IL-2 standard curve in Assay Diluent #12.
Rat IL-2 OneStep ELISA Kit
Rat IL-2 OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | IL-2 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rt |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 2.7%； Inter-assay:3.8% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 9.254 pg/ml |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Recovery | Cell culture supernatant: 113% |
| Assay time | 60 minutes |
| Species reactivity | Rat |
Background
Rat IL-2 (Interleukin-2) is a 15–17 kDa glycoprotein and key T-cell growth factor primarily secreted by activated CD4+ T cells, NK cells, and dendritic cells. It drives T-cell proliferation, Th1 differentiation, and NK cell activity while also supporting regulatory T cell (Treg) function for immune tolerance. Signaling through the IL-2 receptor (IL-2Rαβγ) activates JAK-STAT pathways, promoting cell survival and expansion. Rat IL-2 shares ~70% homology with mouse IL-2 but differs significantly from human IL-2, requiring species-specific detection methods (e.g., ELISA, flow cytometry). It is crucial in research on autoimmunity (e.g., EAE), transplantation, and cancer immunotherapy, with dysregulation linked to immune disorders. Recombinant IL-2 has therapeutic potential but demands rat-specific reagents for accurate study.
Picture
Picture
ELISA
Linearity
The concentrations of Rat IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: Rat spleen stimulated with 5ug/ml Concanavalin A for 2days (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 40.49 ng/mL in stimulated Rat spleen supernatant.
Linearity
The sample is undiluted samples are as follows: Rat spleen for 2days (12.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.09 ng/mL in unstimulated Rat spleen supernatant.
Protocol Diagram
