Standard curve
Example of Mouse IL-4 standard curve in Assay Diluent #1.
Mouse lL-4(Culture Medium) OneStep ELISA Kit
Mouse lL-4(Culture Medium) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-4 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.3%； Inter-assay: 3.7% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.004 pg/mL |
| Range | 4.688 pg/mL – 300 pg/mL |
| Recovery | Cell culture supernatant: 91% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse interleukin-4 (IL-4) is an 18–20 kDa monomeric glycoprotein cytokine primarily produced by Th2 cells, mast cells, basophils, eosinophils, NKT cells, and γδT cells. As a central regulator of type 2 immunity, it drives Th2 differentiation via the JAK-STAT6 pathway upon binding to IL-4 receptor complexes (IL-4Rα paired with γc or IL-13Rα1), inducing transcription factors like GATA3 and c-Maf. Functionally, IL-4 promotes B cell proliferation, class switching to IgG1/IgE, and activation of eosinophils/mast cells, while suppressing Th1 responses. Its roles are context-dependent: IL-4 exerts protective effects in acute lung injury (reducing TNF-α/IL-6 and apoptosis) and cerebral ischemia (attenuating neuroinflammation), yet can exacerbate infections (e.g., mousepox) by suppressing cytolytic lymphocyte responses.
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ELISA
Linearity
The concentrations of Mouse IL-4 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: EL-4 cells stimulated with 10ug/ml PHA and 10 ug/ml PMA for 2 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 290.27 pg/mL in stimulated EL-4 cells supernatant.
Linearity
The sample is undiluted samples are as follows: EL-4 cells culture for 2 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 64.18 pg/mL in unstimulated EL-4 cells supernatant.
Linearity
The concentrations of Mouse IL-4 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse splenocyte were cultured for 3days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 12.23 pg/mL in Mouse splenocytes culture supernatant.
#2 sample is undiluted samples are as follows: An aliquot of the culture medium was removed (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.89 pg/mL in culture medium.
To assess the linearity of the assay, sample was spiked with high concentrations of Mouse IL-4 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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