Standard curve
Example of Mouse IL-6 standard curve in Assay Diluent EC2E1.
Mouse IL-6 OneStep ELISA Kit
Mouse IL-6 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-6 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 6.5%； Inter-assay: 9.8% |
| Sample type | Cell culture supernatant; Serum; Plasma; Cell culture extracts |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.637 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Recovery | Cell culture supernatant: 117% serum: 99% Plasma: 101% Cell culture extracts: 100% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. Mouse IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 in regulating the acute phase response. Mouse IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 has a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.
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ELISA
To assess the linearity of the assay, two samples were spiked with high concentrations of Mouse IL-6 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
