Standard curve
Example of Mouse IL-5 standard curve in Assay Diluent #1.
Mouse IL-5(Culture Medium) OneStep ELISA Kit
Mouse IL-5(Culture Medium) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-5 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 5.1%； Inter-assay: 6.1% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.788 pg/mL |
| Range | 1.95 pg/mL – 125 pg/mL |
| Recovery | Cell culture supernatant: 90% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse Interleukin-5 (IL-5) is a Th2-derived cytokine critical for eosinophil biology. Produced primarily by activated T helper 2 cells and group 2 innate lymphoid cells (ILC2s) in mice, it signals through a heterodimeric receptor (IL-5Rα/βc) to regulate eosinophil differentiation, survival, and activation. This 45-kDa homodimeric glycoprotein drives allergic inflammation and anti-helminth immunity by promoting eosinophil migration to tissues and stimulating immunoglobulin production in B-1 cells. Its pivotal role in eosinophil-mediated pathologies makes it a key therapeutic target in murine asthma models and parasitic infections.
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Picture
ELISA
Linearity
The concentrations of Mouse IL-5 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: EL-4 cells stimulated with 10ug/ml PHA and 10ug/ml PMA for 2days (5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4497.53 pg/mL in stimulated EL-4 cells supernatant.
The sample is undiluted samples are as follows: EL-4 cells culture for 2days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 87.39 pg/mL in unstimulated EL-4 supernatant.
To assess the linearity of the assay, The sample was spiked with high concentrations of Mouse IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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