Standard curve
Example of Mouse IgG2b standard curve in Assay Diluent #1.
Mouse IgG2b Onestep ELISA Kit
Mouse IgG2b Onestep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgG2b |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.7%； Inter-assay: 4.8% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.047 ng/mL |
| Range | 0.47 ng/mL – 30 ng/mL |
| Recovery | Cell culture supernatant: 82% Serum: 107% Plasma: 99% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse IgG2b is another strongly inflammatory antibody isotype that shares functional similarities with IgG2a, though it is genetically distinct. It effectively binds to several activating Fc gamma receptors (FcγRs), though often with different affinities compared to IgG2a. IgG2b is highly competent at triggering key effector functions, including ADCC, ADCP, and complement activation. Its ability to fix complement is generally considered very strong, often on par with or even exceeding that of IgG2a in certain contexts. IgG2b thus plays a crucial role in the humoral immune response by efficiently opsonizing pathogens for clearance and directly lysing target cells through complement-mediated mechanisms.
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Picture
ELISA
Linearity
The concentrations of mouse IgG2b were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: CD-1 mouse serum was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 337.57 ug/mL in CD-1 mouse serum.
#2 sample is undiluted samples are as follows: BABL/C mouse serum was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 42.26 ug/mL in BABL/C mouse serum.
#3 sample is undiluted samples are as follows: C57BL/C mouse serum was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 573.82 ug/mL in C57BL/C mouse serum.
Linearity
The concentrations of mouse IgG2b were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: C57BL/C mouse plasma (EDTA) was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 345.11 ug/mL in C57BL/C mouse plasma (EDTA).
#2 sample is undiluted samples are as follows: C57BL/C mouse plasma (Sodium citrate) was 8000fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 244.16 ug/mL in C57BL/C mouse plasma (Sodium citrate).
Protocol Diagram
