Human IL-6 OneStep ELISA Kit

Catalog Number: S0C3004 Application: ELISA Reactivity: Human Conjugation: Brand: Starter

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$653.00 SGD

Size: 1x96T 5x96T 10x96T

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Product Details

Product Specification

Antigen	IL-6
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	2 to 8 °C as supplied.

Kit

Precision	Intra-assay: 5.2%； Intra-assay: 6.0%
Sample type	Cell culture supernatant, Serum, Plasma
Assay type	Sandwich (quantitative)
Sensitivity	0.14 pg/ml
Range	2.1 pg/ml - 136 pg/ml
Recovery	Cell culture supernatant 90% Serum 98% Plasma 97%
Assay time	60 minutes
Species reactivity	Human
CROSS REACTIVITY	/
INTERFERENCE	/
NIBSC convert	The dose response curve of the International Standard (21/308) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (21/308) units, use the following equation: NIBSC (21/308) approximate value (IU/mL) = 0.142 x OneStep Human IL-6 value (pg/mL).

Background

Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.

Picture

ELISA

Protocol Diagram

Standard curve
Example of human IL-6 standard curve in Assay Diluent #1.

Standard curve
Example of human IL-6 standard curve in Assay Diluent #1S.

Linearity

The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.

#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (0.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 88,750 pg/mL in stimulated human PBMC supernatant.

#2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 46 pg/mL in unstimulated human PBMC supernatant.