Standard curve
Example of Human IL-22 standard curve in Assay Diluent #1.
Human IL-22 OneStep ELISA Kit
Human IL-22 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-22 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay:4.7%； Inter-assay: 5.9% |
| Sample type | Cell culture supernatant, Serum,Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.002 pg/ml |
| Range | 3.125 pg/mL – 200 pg/mL |
| Recovery | Cell culture supernatant: 99% Serum: 95% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
Interleukin-22(IL-22) is a member of the IL10 family of cytokines that mediate cellular inflammatory responses and functions in antimicrobial defense at mucosal surfaces and in tissue repair. This protein also has pro-inflammatory properties and plays a role in in the pathogenesis of several intestinal diseases. IL-22 has a role in the remodeling of the uterine tissue in the inflammatory environment by regulating epithelial-mesenchymal transition markers called E- and N-cadherin. Therefore, IL-22 contributes to the proper regeneration of endometrial layers after inflammation-triggered abortion. Thus, it might have a practical significance to be utilized as a treatment option postpartum (enhanced regeneration function) and in secondary infertility caused by inflammation (enhanced barrier/protector function). IL-22 is a crucial cytokine that regulates host immunity in infectious diseases, including COVID-19 (disease caused by SARS-CoV-2).
Picture
Picture
ELISA
Standard curve
Example of Human IL-22 standard curve in Assay Diluent #12.
Linearity
Linearity
The concentrations of Human IL-22 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 6days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 386.18 pg/mL in stimulated human PBMC supernatant.
Linearity
The sample is undiluted samples are as follows: human PBMC cells culture for 6days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.29 pg/mL in unstimulated human PBMC supernatant.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-22 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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