Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 15-30ng/ml.
Transferrin His Tag Protein, Mouse
Transferrin His Tag Protein, Mouse
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$240 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | TF, DKFZp781D0156, PRO1557, PRO2086 |
| Accession | Q921I1 |
| Amino Acid Sequence | Val20-His697 with His tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 75-85kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & RP-HPLC |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Kong J, Yao Z, Chen J, Zhao Q, Li T, Dong M, Bai Y, Liu Y, Lin Z, Xie Q, Zhang X. Comparative Transcriptome Analysis Unveils Regulatory Factors Influencing Fatty Liver Development in Lion-Head Geese under High-Intake Feeding Compared to Normal Feeding. Vet Sci. 2024 Aug 11;11(8):366. |
Background
Transferrin, also referred to as Serotransferrin, Beta-1 metal-binding globulin, or TF, is an iron-binding blood plasma glycoprotein that regulates the levels of free iron in biological fluids. Despite the fact that iron complexed with transferrin represents less than 0.1% (4 mg) of the total body iron, this pool is the most critical due to its high turnover rate of 25 mg per 24 hours. Transferrin exhibits an extremely high affinity for Fe(III) ions, which diminishes progressively as the pH decreases below neutrality. When not complexed with iron, transferrin is termed “apo-transferrin”.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
The purity of Transferrin His Tag Protein, Mouse is more than 95% determined by RP-HPLC.
