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SKP2 GST Tag Protein, Human

SKP2 GST Tag Protein, Human

Catalog Number: UA085002 Brand: UA BIOSCIENCE
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$483.00 SGD
$483.00 SGD
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Product Details

Product Specification


Species Human
Synonyms FBXL1
Accession Q13309
Amino Acid Sequence

Arg89-Leu424 with GST Tag at the N-Terminus
Expression System E.coli
Molecular Weight

63kDa (Reducing)
Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag GST Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

Schulman BA, Carrano AC, Jeffrey PD, et al. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature. 2000;408(6810):381-386.

Background

SKP2 (S-phase kinase-associated protein 2) is an F-box-containing substrate-recognition subunit of the SCFSKP2 E3 ubiquitin ligase complex (Cullin-1-SKP1-SKP2-Rbx1). Its N-terminal F-box docks onto SKP1, while C-terminal leucine-rich repeats (LRRs) capture phosphorylated targets. By catalysing K48-linked poly-ubiquitylation, SKP2 drives proteasomal degradation of a panel of tumour suppressors and cell-cycle brakes, including p27Kip1, p21Cip1, p57Kip2, FOXO1, BRCA2 and RASSF1A, thereby promoting G1/S progression, DNA replication and survival signalling.

Picture

SDS-PAGE

2μg (R: reducing condition).