The RIPK3 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the RIPK3 protein, ATP and substrate at 25℃ for 120 min, then adding ADP-Glo reagent and reading RLU signal.
RIPK3 Protein
RIPK3 Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | RIP3 |
| Accession | Q9Y572-1 |
| Amino Acid Sequence |
M1-S316 MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSMSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFS |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 60.7 kDa |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij35, pH7.5 |
| Stability & Storage |
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaw cycles.
|
Picture
Picture
Bioactivity
SDS-PAGE
M:MW 1: 3μg RIPK3
