The PLK1 activity was detected using Ultra Luminescence technology. The reaction was performed by
incubating the PLK1 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading
RLU signal with BMG.
PLK1 GST&His Tag Protein, Human
PLK1 GST&His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | PLK |
| Accession | P53350 |
| Amino Acid Sequence | M1-S603(end); N-terminal GST tag, C-terminal 6x His tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 95.9 kDa |
| Purity | >70% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag, GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% Glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
