The PLK1 activity was detected using Ultra Luminescence technology. The reaction was performed by
incubating the PLK1 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading
RLU signal with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | PLK |
Accession | P53350 |
Amino Acid Sequence | M1-S603(end); N-terminal GST tag, C-terminal 6x His tag |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 95.9 kDa |
Purity | >70% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | His Tag, GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% Glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
